RNA

Part:BBa_K4829025:Design

Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal   Group: iGEM23_IISc-Bengaluru   (2023-10-09)


Ribotree optimised sequence for the CDS of BBa_K4829018


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)



Source

To the best of our knowledge, this sequence is not derived from any organism.

References